Journal: BMC Veterinary Research
Article Title: Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8 ∆clpP/apxIIC confers protection against homologous or heterologous strain challenge
doi: 10.1186/s12917-016-0928-9
Figure Lengend Snippet: Levels of IgG antibody ( a ), IgG1 ( b ), and IgG2 ( c ) in the sera of piglets. Blood samples were collected prior to and following immunization on days 0, 21, and 35 from the S-8 △clp△apxIIC immunized groups and PBS control groups, and the antibody responses were determined by indirect ELISA. The results are expressed as the means ± SD
Article Snippet: For determining the IgG isotypes, the sera were added to the S-8 △clpP△apxIIC -coated plates and incubated with mouse anti-pig IgG1 Monoclonal Antibody (Clone K139 3C8, Thermo, United Kingdom) or mouse anti-pig IgG2 Monoclonal Antibody (Clone K68 Ig2, Thermo, United Kingdom), followed by HRP-conjugated goat anti-mouse IgG (Abcam, United Kingdom).
Techniques: Control, Indirect ELISA